Kasama T, Hisano Y, Nakajima M, Handa S, Taki T (1996) Microscale analysis of glycosphingolipids by TLC blotting/secondary ion mass spectrometry: a novel blood group A-active glycosphingolipid and changes in glycosphingolipid expression in rat mammary tumor cells with different metastatic potentials. Taki T, Ishikawa D, Handa S, Kasama T (1995) Direct mass spectrometric analysis of glycosphingolipid transferred to a polyvinylidene difluoride membrane by thin-layer chromatography blotting. Taki T, Kasama T, Handa S, Ishikawa D (1994) A simple and quantitative purification of glycosphingolipids and phospholipids by thin-layer chromatography blotting. Taki T, Handa S, Ishikawa D (1994) Blotting of glycolipids and phospholipids from a high-performance thin-layer chromatogram to a polyvinylidene difluoride membrane. Towbin H, Schoenenberger C, Ball R, Braun DG, Rosenfelder G (1984) Glycosphingolipid-blotting: an immunological detection procedure after separation by thin layer chromatography. Horejsi V, Hrdinka M (2014) Membrane microdomains in immunoreceptor signaling. Hanzal-Bayer ME, Hancock JF (2007) Lipid rafts and membrane traffic. J Clin Invest 110:597–603īrown DA (2006) Lipid rafts, detergent-resistant membranes, and raft targeting signals. Simons K, Ehehalt R (2002) Cholesterol, lipid rafts, and disease. Maeda-Sano K, Gotoh M, Morohoshi T, Someya T, Murofushi H, Murakami-Murofushi K (2014) Cyclic phosphatidic acid and lysophosphatidic acid induce hyaluronic acid synthesis via CREB transcription factor regulation in human skin fibroblasts. Liu Y, Su Y, Wang X (2013) Phosphatidic acid-mediated signaling. Norris PC, Gosselin D, Reichart D, Glass CK, Dennis EA (2014) Phospholipase A2 regulates eicosanoid class switching during inflammasome activation. Matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-TOF MS).The method for identification of individual lipids transferred on the PVDF membrane using matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (TLC-Blot/MALDI-TOF MS) is shown as a functional lipidomics application. Immunodetection with monoclonal antibodies and treatment with lipid-metabolizing enzymes on the lipid-blotted membrane are possible. Individual lipids separated on an HPTLC plate can be transferred to the PVDF membrane quantitatively and also isolated from the lipid-blotted membrane by a one-step purification procedure. This chapter presents the conventional procedures for separating lipid from tissue samples, cultured cells, and serum and the subsequent development of TLC. A simple method for transfer of lipids-including phospholipids, glycolipids, and neutral lipids-from a high performance thin layer chromatography (HPTLC) plate to a polyvinylidene difluoride (PVDF) membrane, TLC-Blot (Far-Eastern Blot), and its biochemical applications are presented.
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